Tissue Specific Expression Levels of Apoptosis Involved Genes Have Correlations with Codon and Amino Acid Usage

Different mechanisms, including transcriptional and post transcriptional processes, regulate tissue specific expression of genes. In this study, we report differences in gene/protein compositional features between apoptosis involved genes selectively expressed in human tissues. We found some correlations between codon/amino acid usage and tissue specific expression level of genes. The findings can be significant for understanding the translational selection on these features. The selection may play an important role in the differentiation of human tissues and can be considered for future studies in diagnosis of some diseases such as cancer.

6-Methoxy Podophyllotoxin induces Apoptosis via inhibition of TUBB3 and TOPIIA Gene expressions in 5637 and K562 Cancer cell lines

Objective: Podophyllotoxin [PTOX], a natural compound in numerous plants, contains remarkable biological properties that include anti-tumor, anti-viral such as anti-human im-munodeficiency virus [HIV] activities. In order to avoid its adverse effects, various compounds have been derived from PTOX. 6-methoxy PTOX [MPTOX] is one of the natural PTOX derivatives with an extra methoxy group. MPTOX is mostly isolated from the Linum species. This study has sought to determine the biological effects of MPTOX on cancer cell lines, 5637 and K562

Materials and Methods: In this experimental study, we treated the 5637 and K562 cancer cell lines with MPTOX in a dose- and time-dependent manner. Apoptosis was examined by flow cytometry and viability rate was analyzed by the MTT assay. Expressions of the tubulin [TUBB3] and topoisomerase II [TOPIIA] genes were determined by real-time polymerase chain reaction [PCR]

Results: Treatment with MPTOX led to significant induction of apoptosis in cancer cells compared to control cells. Gene expression analysis showed reduced levels of TUBB3 and TOPIIA mRNA following MPTOX treatment

Conclusion: MPTOX inhibited TUBB3 and TOPIIA gene expression and subsequently induced cell death through apoptosis. These results suggested that MPTOX could be considered a potential anti-tumor agent